Naruse T, Fukasawa T, Miyakawa Y
Lab Invest. 1975 Aug;33(2):141-6.
The laboratory counterpart of tubular antigen-mediated membranous glomerulonephritis in humans was produced in rats by a single injection of homologous nephritogenic tubular antigen with adjuvant. The rat developed membranous glomerulonephritis with typical clinicopathologic features of human nephritis mediated by the tubular antigen, i.e., massive proteinuria and diffuse thickening of glomerular basement membranes due to deposition of tubular antigen-antibody complexes which were demonstrated by immunofluorescent technique. The nephritogenic tubular antigen was solubilized by pronase digestion, further purified by gel filtration, and demonstrated to have the same physicochemical properties as the human tubular antigen which was found in the deposits together with beta1C- and immunoglobulins in the glomeruli of patients with membranous glomerulonephritis.
通过单次注射同源致肾炎性肾小管抗原加佐剂，在大鼠中产生了人类肾小管抗原介导的膜性肾小球肾炎的实验对应物。大鼠发生了膜性肾小球肾炎，具有由肾小管抗原介导的人类肾炎的典型临床病理特征，即大量蛋白尿以及由于肾小管抗原 - 抗体复合物沉积导致的肾小球基底膜弥漫性增厚，免疫荧光技术证实了这些复合物的存在。致肾炎性肾小管抗原经链霉蛋白酶消化溶解，通过凝胶过滤进一步纯化，并证明其具有与人类肾小管抗原相同的物理化学性质，在膜性肾小球肾炎患者的肾小球沉积物中，该抗原与β1C和免疫球蛋白一起被发现。
