Slonek J E, Peng G W, Chiou W L
J Pharm Sci. 1978 Oct;67(10):1462-4. doi: 10.1002/jps.2600671037.
A rapid and simple high-pressure liquid chromatographic microanalytical method was developed for the determination of clinically encountered plasma phenytoin levels. This method is accurate down to about 1 microgram of phenytoin/ml of plasma and requires as little as 10 microliter of sample. Total analysis time is about 10 min. The method involves deproteinizing with acetonitrile followed by monitoring the deproteinized sample at 254 nm. Phenytoin's primary metabolite in humans, 5-(p-hydroxyphenyl)-5-phenylhydantoin, also can be quantitated when present in moderately high clinically encountered concentrations. Plasma profiles of phenytoin and its metabolite were followed with time after an intravenous bolus injection to a rabbit.
已开发出一种快速简便的高压液相色谱微量分析方法，用于测定临床中遇到的血浆苯妥英水平。该方法可精确测定低至约1微克苯妥英/毫升血浆的含量，且所需样品量低至10微升。总分析时间约为10分钟。该方法包括用乙腈进行蛋白沉淀，然后在254纳米处监测蛋白沉淀后的样品。当人体中苯妥英的主要代谢产物5-（对羟基苯基）-5-苯基乙内酰脲以临床中遇到的中等高浓度存在时，也可进行定量分析。在给一只兔子静脉推注后，随时间跟踪苯妥英及其代谢产物的血浆分布情况。
