Gadalla M A, Peng G W, Chiou W L
J Pharm Sci. 1978 Jun;67(6):869-71. doi: 10.1002/jps.2600670641.
A rapid and simple high-pressure liquid chromatographic method was developed for the simultaneous determination of plasma levels of procainamide and its major metabolite, N-acetonitrile, and the supernate was chromatographed on a cation-exchange column. The assay can be carried out on as little as 20 microliter of plasma and requires only about 7 min for each sample. No interference was found in plasma samples from cardiac patients receiving procainamide. This method is simple, fast, and useful for routine therapeutic monitoring and for pharmacokinetic studies procainamide and its metabolite.
建立了一种快速简便的高压液相色谱法，用于同时测定血浆中普鲁卡因胺及其主要代谢产物N-乙酰乙胺的水平，上清液在阳离子交换柱上进行色谱分析。该测定法只需20微升血浆即可进行，每个样品仅需约7分钟。在接受普鲁卡因胺治疗的心脏病患者的血浆样本中未发现干扰。该方法简单、快速，可用于普鲁卡因胺及其代谢产物的常规治疗监测和药代动力学研究。
