Smith S H, Harris L S, Uwaydah I M, Munson A E
J Pharmacol Exp Ther. 1978 Oct;207(1):165-70.
Delta-9-Tetrahydrocannabinol (delta-9-THC) a psychoactive constituent of marihuana, has been shown in previous studies to suppress humoral and cell-mediated immunity. The objective of this study was to determine whether structural modification of delta-9-THC would result in a more potent immunosuppressant agent devoid of central nervous system activity. The ED50 for reduction of the humoral immune response to sheep erythrocytes as measured by spleen plaque forming cells/10(6) spleen cells was 70 mg/kg for delta-9-THC. Delta-8-THC, 1-methyl delta-8-THC and abnormal delta-8-THC were more potent with ED50 values of 14, 13 and 8 mg/kg, respectively. Cell-mediated immunity was evaluated by a delayed-type hypersensitivity response to sheep erythrocytes. At doses of 100 mg/kg s.c. for 4 days, the cannabinoids suppressed the delayed-type hypersensitivity response from 35 to 64%. These data show that the cannabinoids can cause immunosuppression which is not related to central nervous system activity since 1-methyl delta-8-THC and abnormal delta-8-THC have minimal central nervous system activity.
Δ⁹-四氢大麻酚（Δ⁹-THC）是大麻中的一种精神活性成分，先前的研究表明它能抑制体液免疫和细胞介导的免疫。本研究的目的是确定Δ⁹-THC的结构修饰是否会产生一种更有效的免疫抑制剂，且无中枢神经系统活性。以脾空斑形成细胞/10⁶脾细胞衡量，Δ⁹-THC降低对绵羊红细胞体液免疫反应的半数有效剂量（ED50）为70mg/kg。Δ⁸-THC、1-甲基-Δ⁸-THC和异常Δ⁸-THC的效力更强，ED50值分别为14mg/kg、13mg/kg和8mg/kg。通过对绵羊红细胞的迟发型超敏反应评估细胞介导的免疫。皮下注射剂量为100mg/kg，持续4天，大麻素将迟发型超敏反应抑制了35%至64%。这些数据表明，大麻素可引起免疫抑制，这与中枢神经系统活性无关，因为1-甲基-Δ⁸-THC和异常Δ⁸-THC的中枢神经系统活性极小。
