Lithgow T, Barr G A
Brain Res. 1981 Sep;254(2):315-20. doi: 10.1016/0165-3806(81)90040-7.
Presented are methods of implanting chronically indwelling electrodes or cannulas into brain infant rats. Anesthesized pups are restrained in a flexible alginate mold that is place in the stereotaxic instrument. Details of electrode implant are given. We routinely use these methods to accurately place electrodes into subcortical structures of 3- to 15-day-old pups with less than 2% loss of the implant.
本文介绍了将慢性植入电极或套管植入幼鼠大脑的方法。将麻醉后的幼崽固定在置于立体定位仪中的柔性藻酸盐模具中。文中给出了电极植入的详细信息。我们经常使用这些方法将电极精确植入3至15日龄幼崽的皮层下结构,植入成功率低于2%。