Nesterova R N
Biull Eksp Biol Med. 1977 Jul;84(7):124-6.
The author suggests an express method of radioimmunological determination of murine and human alpha-fetoprotein with the use of electrophoretic separation of immune-bound and free antigen. The method is comparatively simple, it permits to analyze up to 50 samples in the course of 5 hours. Behaviour of the antibody-antigen system in drawing the standard inhibition curves was analyzed. Inhibition of the antibody-antigen reaction in the zone of low and great concentrations was revealed. The effect described requires further study.
作者提出了一种利用免疫结合抗原和游离抗原的电泳分离来快速放射免疫测定小鼠和人甲胎蛋白的方法。该方法相对简单,在5小时内可分析多达50个样品。分析了抗体-抗原系统在绘制标准抑制曲线时的行为。揭示了在低浓度和高浓度区域抗体-抗原反应的抑制情况。所述效应需要进一步研究。