Stanley J R, Alvarez O M, Bere E W, Eaglstein W H, Katz S I
J Invest Dermatol. 1981 Aug;77(2):240-3. doi: 10.1111/1523-1747.ep12480082.
Bullous pemphigoid (BP) antigen, laminin, and type IV collagen, 3 distinct antigens of basement membrane, were studied by indirect immunofluorescence in the epidermal-dermal junction of re-epithelializing wounds. Partial thickness wounds were made with a dermatome in the skin of white Yorkshire pigs. After 2 or 3 days, the wound site and the surrounding normal skin were excised and cryostat sections were studied using BP sera as well as whole antisera and affinity purified antibodies to laminin and type IV collagen. Laminin and type IV collagen were detected in the basement membrane zone of normal epidermis and at the re-epithelializing epidermal-dermal junction for a variable distance into the healing wound but both were absent from the more distal migrating epidermis. In contrast, BP antigen extended from the basement membrane zone of normal skin throughout the entire epidermal-dermal junction of dermis. These results suggest that in the re-epithelization of superficial wounds laminin and type IV collagen are not present in the initial epidermal-dermal interaction of the migrating epithelium but that BP antigen may be important in this early interaction.
通过间接免疫荧光法，研究了大疱性类天疱疮（BP）抗原、层粘连蛋白和IV型胶原这3种基底膜的不同抗原，它们存在于再上皮化伤口的表皮-真皮交界处。用皮肤刀在白色约克郡猪的皮肤上制造部分厚度的伤口。2或3天后，切除伤口部位及周围正常皮肤，使用BP血清以及针对层粘连蛋白和IV型胶原的全抗血清和亲和纯化抗体对冰冻切片进行研究。在正常表皮的基底膜区以及再上皮化的表皮-真皮交界处，在愈合伤口内一定距离处可检测到层粘连蛋白和IV型胶原，但在更远端迁移的表皮中两者均不存在。相比之下，BP抗原从正常皮肤的基底膜区延伸至整个真皮的表皮-真皮交界处。这些结果表明，在浅表伤口的再上皮化过程中，迁移上皮的初始表皮-真皮相互作用中不存在层粘连蛋白和IV型胶原，但BP抗原可能在这种早期相互作用中起重要作用。
