Zhukov-Verezhnikov N N, Drozhennikov V A, Stoliarova L G, Orlova E B, Perevezentseva O S
Biull Eksp Biol Med. 1977 Jul;84(7):68-70.
Bacillus subtilis transformation was conducted in the abdominal cavity of mice. The frequency of transformation was considerable decreased when bovine DNA-ase 1 (3-- 5microgram) was injected intraperitoneally to these animals. Immune rabbit gamma-globulins containing antibodies to bovine DNA-ase 1 inhibited in vivo the activity of DNA-ase 1, protected transforming DNA from the hydrolyzing effect of this enzyme. This model can be utilized in search for ways to preserve DNA injected into the animal organism for the purpose of genetical engineering.
枯草芽孢杆菌转化在小鼠腹腔内进行。当向这些动物腹腔内注射牛DNA酶1（3 - 5微克）时，转化频率显著降低。含有抗牛DNA酶1抗体的免疫兔γ球蛋白在体内抑制DNA酶1的活性，保护转化DNA免受该酶的水解作用。该模型可用于寻找为基因工程目的而注入动物机体的DNA的保存方法。
