大肠杆菌中正常色氨酸转运RNA对UGA的抑制作用：密码子上下文效应
UGA suppression by normal tRNA Trp in Escherichia coli: codon context effects.
作者信息
Engelberg-Kulka H
出版信息
Nucleic Acids Res. 1981 Feb 25;9(4):983-91. doi: 10.1093/nar/9.4.983.
Abstract
The nucleotide sequences at the 3' side of in-phase UGA termination codons in mRNAs of various prokaryotic genes were re-examined. An adenine (A) residue is found to be adjacent to the 3' side of UGA in mRNAs which code for readthrough proteins by the suppression of UGA by normal Escherichia coli tRNA Trp. It is suggested that the nature of the nucleotide following a UGA codon determines whether the UGA signals inefficiently or efficiently the termination of polypeptide chain synthesis: an A residue at this position permits the UGA readthrough process.
摘要
对各种原核基因mRNA中同相位UGA终止密码子3'端的核苷酸序列进行了重新研究。发现在通过正常大肠杆菌色氨酸tRNA抑制UGA而编码通读蛋白的mRNA中，腺嘌呤（A）残基与UGA的3'端相邻。有人提出，UGA密码子之后的核苷酸性质决定了UGA是低效还是高效地发出多肽链合成终止信号：该位置的A残基允许UGA通读过程。
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