Silverman L M, Chapman J F, Jones M E, Dermer G B, Pullano T, Tökes Z A
Prostate. 1981;2(1):109-19. doi: 10.1002/pros.2990020111.
Creatine kinase BB has been described to be elevated in the serum of patients with prostate cancer. The incidence of abnormal serum levels correlates with the degree of differentiation of the tumor; that is, the more poorly differentiated tumors are associated most frequently with elevated CK-BB levels. We have recently described two additional findings which involve CK-BB in prostate cancer: 1) the finding of abnormally migrating CK-BB bands reported to be IgG-CK-BB complexes in several Stage D patients and in 2/3 Stage B patients following 125I implants, and 2) significant differences between CK-BB purified from prostatic fluid from CK-BB of brain origin. In particular, we find two bands of prostatic CK-BB following purification as compared to a single band from brain, when the same purification protocol is used. While further characterization of these differences is proceeding, the two bands of CK-BB are being used to develop a prostate-specific antiserum for use in immunoassays to detect prostate cancer in conjunction with other traditional markers such as prostatic acid phosphatase. If serum detection of tumor markers is to be efficacious, tumor marker panels such as CK-BB and prostatic acid phosphatase may provide a significant advantage over individual markers.
已有报道称，前列腺癌患者血清中的肌酸激酶BB（CK-BB）水平会升高。血清水平异常的发生率与肿瘤的分化程度相关；也就是说，分化程度越低的肿瘤，其CK-BB水平升高的情况最为常见。我们最近还描述了另外两个与前列腺癌中CK-BB相关的发现：1）在几名D期患者以及2/3的B期患者接受¹²⁵I植入后，发现了异常迁移的CK-BB条带，据报道这些条带为IgG-CK-BB复合物；2）从前列腺液中纯化得到的CK-BB与脑源性CK-BB之间存在显著差异。具体而言，当采用相同的纯化方案时，我们发现纯化后的前列腺CK-BB有两条条带，而脑源性CK-BB只有一条条带。在对这些差异进行进一步表征的同时，这两条CK-BB条带正被用于开发一种前列腺特异性抗血清，用于免疫测定，以便与其他传统标志物（如前列腺酸性磷酸酶）联合检测前列腺癌。如果血清肿瘤标志物检测要有效，像CK-BB和前列腺酸性磷酸酶这样的肿瘤标志物组合可能比单个标志物具有显著优势。
