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鼠伤寒沙门氏菌磷酸烯醇丙酮酸依赖性葡萄糖磷酸转移酶系统中IIIGlc的分离。

Isolation of IIIGlc of the phosphoenolpyruvate-dependent glucose phosphotransferase system of Salmonella typhimurium.

作者信息

Scholte B J, Schuitema A R, Postma P W

出版信息

J Bacteriol. 1981 Oct;148(1):257-64. doi: 10.1128/jb.148.1.257-264.1981.

DOI:10.1128/jb.148.1.257-264.1981
PMID:7026533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216188/
Abstract

We report a procedure for the isolation of IIIglc of Salmonella typhimurium, a protein component of the phosphoenolpyruvate-dependent sugar phosphotransferase system. IIIGlc is a soluble protein with a molecular weight of 21,000, as determined by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified protein is involved in the phosphoenolpyruvate-dependent phosphorylation of methyl alpha-glucoside in vitro. Its affinity for octyl-Sepharose may be an indication of the partial hydrophobic nature of IIIGlc. A specific antiserum against purified IIIGlc was prepared. Growth on different carbon sources did not affect the synthesis of IIIGlc, as determined by quantitative immunoelectrophoresis. Mutations which lower the adenosine 3',5'-phosphate level, such as cya and pts, do not alter the IIIGlc level. The closely related enteric bacteria Escherichia coli and Klebsiella aerogenes contain a protein factor which is closely related to IIIGlc of S. typhimurium, whereas Staphylococcus aureus does not.

摘要

我们报告了一种分离鼠伤寒沙门氏菌IIIglc的方法，IIIglc是磷酸烯醇丙酮酸依赖性糖磷酸转移酶系统的一种蛋白质成分。通过凝胶过滤和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，IIIglc是一种分子量为21,000的可溶性蛋白质。纯化后的蛋白质在体外参与磷酸烯醇丙酮酸依赖性的α-甲基葡萄糖苷磷酸化过程。它对辛基琼脂糖的亲和力可能表明IIIglc具有部分疏水性质。制备了针对纯化IIIglc的特异性抗血清。通过定量免疫电泳测定，在不同碳源上生长不影响IIIglc的合成。降低3',5'-磷酸腺苷水平（如cya和pts突变）的突变不会改变IIIglc的水平。密切相关的肠道细菌大肠杆菌和产气克雷伯菌含有一种与鼠伤寒沙门氏菌IIIglc密切相关的蛋白质因子，而金黄色葡萄球菌则没有。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/812c790aa3f0/jbacter00263-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/955a9b2909aa/jbacter00263-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/df7601ef5f38/jbacter00263-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/a76457ec0922/jbacter00263-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/812c790aa3f0/jbacter00263-0270-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/955a9b2909aa/jbacter00263-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/df7601ef5f38/jbacter00263-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/a76457ec0922/jbacter00263-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6990/216188/812c790aa3f0/jbacter00263-0270-a.jpg

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