Dougherty K A, Cockett A T, Urry R L
J Urol. 1978 Oct;120(4):425-6. doi: 10.1016/s0022-5347(17)57213-4.
The effect of increasing concentrations of amylase on the percentage of active spermatozoa, the quality of their motility and the percentage of viable cells was studied in semen samples in vitro. The amount of amylase needed to liquefy viscous semen samples in vitro also was determined. The percentage of active spermatozoa and viable cells, and the quality of sperm motility were altered in relationship to the amylase levels. Significant decreases in these parameters compared to control values were seen at the higher concentrations of amylase. The lowest level of amylase did not alter these parameters significantly and was sufficient to liquefy 80 per cent of the viscous semen samples. Amylase appears to be effective at low concentrations for use in liquefying viscous semen samples, thus making them easier to analyze during routine semen examination. The level of amylase used and the interval between addition and analysis must be controlled carefully.
在体外对精液样本研究了淀粉酶浓度增加对活动精子百分比、精子活力质量和活细胞百分比的影响。还测定了体外液化黏稠精液样本所需的淀粉酶量。活动精子和活细胞的百分比以及精子活力质量随淀粉酶水平而改变。在较高淀粉酶浓度下，与对照值相比，这些参数显著降低。最低水平的淀粉酶未显著改变这些参数，且足以液化80%的黏稠精液样本。淀粉酶在低浓度下似乎可有效用于液化黏稠精液样本，从而使其在常规精液检查期间更易于分析。所使用的淀粉酶水平以及添加与分析之间的间隔必须仔细控制。
