Dougherty K A, Cockett A T, Urry R L
Fertil Steril. 1976 May;27(5):541-8.
Concentrations of 10(-2), 10(-3), 10(-4), 10(-5), or 10(-6) M caffeine and theophylline were added to semen samples to determine the effects of these compounds on several parameters of semen quality. Semen samples were incubated at 37 degrees C and observed 0.5,1,2,4, and 6 hours after addition of the respective compounds. Semen quality was determined by estimating the percentage of active spermatozoa, by estimating sperm motility, and by counting the number of viable sperm at each time interval. Neither of the compounds, at any of the dose levels tested, had any significantly different effects on the quality of sperm motility or the percentage of active or viable spermatozoa, although a few samples responded favorably to these compounds. Noted improvements in semen quality did not appear to be dependent on the initial semen quality. The data contradict previous reports and suggest that methyl xanthines do not have beneficial effects on the majority of semen samples obtained from men seen at a fertility evaluation clinic.
将浓度为10⁻²、10⁻³、10⁻⁴、10⁻⁵或10⁻⁶ M的咖啡因和茶碱添加到精液样本中，以确定这些化合物对精液质量的几个参数的影响。精液样本在37℃下孵育，并在添加相应化合物后的0.5、1、2、4和6小时进行观察。通过估计活动精子的百分比、估计精子活力以及在每个时间间隔计数活精子的数量来确定精液质量。在所测试的任何剂量水平下，这两种化合物对精子活力质量或活动精子或活精子的百分比均无任何显著差异的影响，尽管少数样本对这些化合物有良好反应。精液质量的明显改善似乎并不取决于初始精液质量。这些数据与先前的报告相矛盾，并表明甲基黄嘌呤对从生育评估诊所就诊的男性获得的大多数精液样本没有有益影响。
