Bortolussi R, Faulkner G, Lee S H, Ozere R
Pediatr Res. 1981 Sep;15(9):1287-92. doi: 10.1203/00006450-198109000-00012.
Inasmuch as human monocytes are able to kill Candida albicans (C. albicans) only through oxidative pathways, which also produce chemiluminescence (CL), CL was used to assess the ability of polymorphonuclear neutrophils and monocytes to phagocytose and kill C. albicans in a 12-year-old girl with chronic mucocutaneous candidiasis. In contrast to normal mononuclear cells (monocytes and lymphocytes), mononuclear cells from the patient did not respond with a CL burst when mixed with opsonized C. albicans (peak CL, 55 versus 105 cpm X 10(-3) for control). Phagocytosis of C. albicans by monocytes, assessed by electron microscopy, was normal. The patient's mononuclear cells did produce CL when mixed with Candida parapsilosis (peak CL, 68 versus 72 cpm X 10(-3) for control) or zymosan (peak CL, 149 versus 180 cpm X 10(-3) for control). Myeloperoxidase activity in monocytes assessed by light microscopy was normal. However, peroxidase activity in the patient's monocytes persisted in glass-adherent monocyte-macrophages after 5 days incubation, suggesting that her cells may mature poorly. In contrast to the poor CL response of monocytes to C. albicans, polymorphonuclear neutrophils from the patient had increased CL (peak, 858 versus 458 cpm X 10(-3) for control). Also, the patient's serum showed increased opsonic activity for C. albicans (peak, 1800 versus 1100 cpm X 10(-3) for control).
由于人类单核细胞仅能通过氧化途径杀死白色念珠菌（白色假丝酵母），而氧化途径也会产生化学发光（CL），因此利用CL来评估一名患有慢性黏膜皮肤念珠菌病的12岁女孩的多形核中性粒细胞和单核细胞吞噬及杀死白色念珠菌的能力。与正常单核细胞（单核细胞和淋巴细胞）不同，该患者的单核细胞与经调理的白色念珠菌混合时不会产生CL爆发（峰值CL，对照组为105 cpm×10⁻³，患者组为55 cpm×10⁻³）。通过电子显微镜评估，单核细胞对白色念珠菌的吞噬作用正常。当该患者的单核细胞与近平滑念珠菌混合时（峰值CL，对照组为72 cpm×10⁻³，患者组为68 cpm×10⁻³）或与酵母聚糖混合时（峰值CL，对照组为180 cpm×10⁻³，患者组为149 cpm×10⁻³）确实会产生CL。通过光学显微镜评估，单核细胞中的髓过氧化物酶活性正常。然而，患者单核细胞中的过氧化物酶活性在玻璃贴壁单核细胞 - 巨噬细胞孵育5天后仍然存在，这表明她的细胞可能成熟不良。与单核细胞对白色念珠菌的CL反应不佳形成对比的是，该患者的多形核中性粒细胞的CL增加（峰值，对照组为458 cpm×10⁻³，患者组为858 cpm×10⁻³）。此外，患者血清对白色念珠菌的调理活性增加（峰值，对照组为1100 cpm×10⁻³，患者组为1800 cpm×10⁻³）。
