Gheorghiu M, Lagranderie M, Bredèche M
Ann Microbiol (Paris). 1981 May-Jun;132(3):307-18.
A new multiple puncture instrument with interchangeable pieces has been devised. It permits the application of 12 or 28 cutaneous punctures, depending on the age of the patient (child). Fresh concentrated (50 mg/ml) BCG vaccine was distributed either into neutral glass ampoules or into neutral polypropylene tubes. No difference in viability was observed after storage at 4 degrees C and in a series of 10 batches of vaccine, a good survival (about 50%) was found after three months. Also, survival was identical among 9 batches of vaccine, whether it was resuspended in a protective solution containing human albumin plus 5% glycerol or in 6% glycerol only. The immunogenicity of the fresh concentrated vaccine, when applied with the new multiple puncture device, was studied using different tests: --the growth of BCG in organs of mice, --the survival time after virulent challenge of vaccinated versus unvaccinated mice, --tuberculin-delayed hypersensitivity of vaccinated guinea-pigs. The immunogenic potency has been found similar to that observed using other methods of BCG vaccination. These experimental results confirm the merit of the new method of vaccination using the multiple puncture device.
一种带有可互换部件的新型多点穿刺器械已被设计出来。根据患者（儿童）年龄，它可进行12次或28次皮肤穿刺。新鲜浓缩（50毫克/毫升）卡介苗被分装到中性玻璃安瓿或中性聚丙烯管中。在4℃储存后，未观察到活力差异，并且在一系列10批疫苗中，三个月后发现有良好的存活率（约50%）。此外，9批疫苗无论是重新悬浮于含人白蛋白加5%甘油的保护溶液中还是仅悬浮于6%甘油中，存活率都是相同的。使用新的多点穿刺装置接种新鲜浓缩疫苗时，通过不同测试研究了其免疫原性：——卡介苗在小鼠器官中的生长情况，——接种疫苗与未接种疫苗的小鼠在受到强毒株攻击后的存活时间，——接种疫苗的豚鼠的结核菌素迟发型超敏反应。已发现免疫原性效力与使用其他卡介苗接种方法时观察到的相似。这些实验结果证实了使用多点穿刺装置进行疫苗接种新方法的优点。
