Ridley S P, Oeschger M P
J Bacteriol. 1982 Nov;152(2):736-46. doi: 10.1128/jb.152.2.736-746.1982.
An Escherichia coli strain carrying an amber mutation (UAG) in rpoC, the gene encoding the beta prime subunit of RNA polymerase, was isolated after mutagenesis with nitrosoguanidine. The mutation was moved into an unmutagenized strain carrying the supD43,74 allele, which encodes a temperature-sensitive su1 amber suppressor, and sue alleles, which enhance the efficiency of the suppressor. In this background, beta prime is not synthesized at high temperature. Suppression of the mutation by the non-temperature-sensitive amber suppressor su1+ yields a protein which is functional at all temperatures examined (30, 37, and 42 degrees C).
在用亚硝基胍诱变后，分离出了一株大肠杆菌菌株，该菌株在编码RNA聚合酶β′亚基的rpoC基因中携带一个琥珀突变（UAG）。该突变被转移到一个未诱变的菌株中，该菌株携带supD43,74等位基因，其编码一种温度敏感的琥珀抑制基因su1，以及sue等位基因，后者可提高抑制基因的效率。在此背景下，β′在高温下不合成。非温度敏感的琥珀抑制基因su1+对该突变的抑制产生了一种在所有检测温度（30、37和42摄氏度）下均有功能的蛋白质。
