Hao K, Takeuchi T, Sato S, Sugimura T
Clin Chim Acta. 1977 Aug 15;79(1):75-80. doi: 10.1016/0009-8981(77)90463-6.
Addition of albumin, gamma-globulin, alpha-casein or submaxillary mucin to the assay system for chromogenic measurement of human or rat amylase with blue starch increased the amylase activity, albumin having the most effect. These proteins seemed to increase the activity by protecting amylase from inactivation. Amylase activity was higher in urine samples showing proteinuria than in urine samples without detectable protein. It is concluded that amylase assay is more reliable when a final concentration of albumin of 1 mg per ml is added at the dilution step and at the incubation step.
在使用蓝色淀粉对人或大鼠淀粉酶进行比色测定的检测系统中添加白蛋白、γ-球蛋白、α-酪蛋白或颌下粘蛋白，会增加淀粉酶活性，其中白蛋白的作用最为显著。这些蛋白质似乎通过保护淀粉酶不被灭活来提高其活性。蛋白尿尿液样本中的淀粉酶活性高于未检测到蛋白质的尿液样本。得出的结论是，在稀释步骤和孵育步骤添加终浓度为每毫升1毫克的白蛋白时，淀粉酶测定更为可靠。
