Khansari N, Segre M, Segre D
J Immunol. 1981 Nov;127(5):1889-93.
Mice infected with Plasmodium berghei have a depressed immune response to a variety of antigens. We report the extraction, purification, and characterization of a soluble immunosuppressive substance derived from P. berghei-infected mouse blood. A crude extract, prepared by solubilization of infected erythrocytes in a Parr cell disruption bomb, reduced the anti-DNP PFC response of mice injected with the extract 1 day before immunization. Purification of the immunosuppressant was accomplished by precipitation with 50% saturated ammonium sulfate followed by chromatography on Sephadex G-150 in the presence of 6 M guanidine hydrochloride. The immunosuppressive activity was recovered in the last fraction eluted from the Sephadex G-150 column that contained low m.w. components. The activity was abrogated by trypsin digestion, but not by periodate oxidation. Volume for volume, the purified immunosuppressant had a 100-fold greater activity than the crude extract from which it was derived. It suppressed the response to the T-dependent antigens DNP-KLH and SRBC, but not to the T-independent antigen DNP-Ficoll.
感染伯氏疟原虫的小鼠对多种抗原的免疫反应会受到抑制。我们报告了从感染伯氏疟原虫的小鼠血液中提取、纯化和鉴定一种可溶性免疫抑制物质的过程。通过在帕尔细胞破碎弹中溶解感染的红细胞制备的粗提物，降低了在免疫前1天注射该提取物的小鼠的抗二硝基苯酚（DNP）空斑形成细胞（PFC）反应。免疫抑制剂的纯化是通过用50%饱和硫酸铵沉淀，然后在6 M盐酸胍存在下在葡聚糖G - 150上进行色谱分离来完成的。免疫抑制活性在从葡聚糖G - 150柱洗脱的最后一个含有低分子量成分的级分中恢复。该活性被胰蛋白酶消化消除，但未被高碘酸盐氧化消除。按体积计算，纯化后的免疫抑制剂的活性比其来源的粗提物高100倍。它抑制对T细胞依赖性抗原DNP - 钥孔戚血蓝蛋白（KLH）和绵羊红细胞（SRBC）的反应，但不抑制对T细胞非依赖性抗原DNP - 聚蔗糖的反应。
