DNA协同因子的测定
Determination of DNA cooperativity factor.
作者信息
Amirikyan B R, Vologodskii A V
出版信息
Nucleic Acids Res. 1981 Oct 24;9(20):5469-82. doi: 10.1093/nar/9.20.5469.
Abstract
The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.
摘要
本文介绍了对colE1 DNA区域位于DNA螺旋内部及其末端时熔点差异的测量。基于螺旋-线圈转变严格理论的计算与0.2M Na+（完全可逆熔解条件）下的实验数据直接比较，得到协同因子σ的值为2.5 - 5×10⁻⁵。我们讨论了DNA熔解的可逆性以及将“全或无”概念应用于DNA区域熔解的可能性。
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本文引用的文献
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