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1
Determination of DNA cooperativity factor.DNA协同因子的测定
Nucleic Acids Res. 1981 Oct 24;9(20):5469-82. doi: 10.1093/nar/9.20.5469.
2
Differential scanning calorimetric and theoretical studies of ColE1 DNA.大肠杆菌E1质粒DNA的差示扫描量热法及理论研究
Nucleic Acids Symp Ser. 1986(17):203-6.
3
Relationship between helix-coil transition and gene organization of ColE1 plasmid DNA. Differential scanning calorimetric and theoretical studies.ColE1质粒DNA的螺旋-卷曲转变与基因组织之间的关系。差示扫描量热法和理论研究。
J Mol Biol. 1987 Apr 20;194(4):691-8. doi: 10.1016/0022-2836(87)90246-4.
4
Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization.质粒ColE1 DNA的平衡熔解：电子显微镜观察
Nucleic Acids Res. 1980 Sep 25;8(18):4165-84. doi: 10.1093/nar/8.18.4165.
5
The ionic strength dependence of the cooperativity factor for DNA melting.DNA解链协同因子对离子强度的依赖性。
J Biomol Struct Dyn. 1987 Aug;5(1):119-26. doi: 10.1080/07391102.1987.10506380.
6
[Nucleoprotein melting. I. Theory of helix-coil transition of DNA in the presence of proteins with cooperative character of interaction under conditions of reversible binding].
Mol Biol (Mosk). 1976 Nov-Dec;10(6):1332-40.
7
[Theory of DNA melting in the interval of B-A transition].
Biofizika. 1983 Sep-Oct;28(5):880-2.
8
High resolution thermal denaturation analyses of small sequenced DNA restriction fragments containing Escherichia coli lactose genetic control loci.
J Biol Chem. 1979 Oct 25;254(20):10128-34.
9
Early melting of supercoiled DNA topoisomers observed by TGGE.通过TGGE观察到超螺旋DNA拓扑异构体的早期解旋
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Determination of melting temperature and temperature melting range for DNA with multi-peak differential melting curves.具有多峰差示熔解曲线的DNA熔解温度和熔解温度范围的测定
Anal Biochem. 2015 Jun 15;479:28-36. doi: 10.1016/j.ab.2015.01.018. Epub 2015 Jan 30.

引用本文的文献

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Euler buckling and nonlinear kinking of double-stranded DNA.双链 DNA 的 Euler 屈曲和非线性扭结。
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Torque spectroscopy of DNA: base-pair stability, boundary effects, backbending, and breathing dynamics.DNA 的扭矩光谱学：碱基对稳定性、边界效应、反向弯曲和呼吸动力学。
Phys Rev Lett. 2013 Apr 26;110(17):178103. doi: 10.1103/PhysRevLett.110.178103. Epub 2013 Apr 25.
4
Torque measurements reveal sequence-specific cooperative transitions in supercoiled DNA.扭矩测量揭示超螺旋 DNA 中的序列特异性协同转变。
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6106-11. doi: 10.1073/pnas.1113532109. Epub 2012 Apr 2.
5
Microscopic mechanism for experimentally observed anomalous elasticity of DNA in two dimensions.二维DNA实验观测到的反常弹性的微观机制
Biophys J. 2009 Jun 3;96(11):4464-9. doi: 10.1016/j.bpj.2009.03.035.
6
Sequence-dependent base pair opening in DNA double helix.DNA双螺旋中依赖序列的碱基对打开
Biophys J. 2006 May 1;90(9):3091-9. doi: 10.1529/biophysj.105.078774. Epub 2006 Feb 24.
7
Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application.部分变性DNA的凝胶电泳。阻滞效应：其分析与应用。
Nucleic Acids Res. 1982 Aug 11;10(15):4813-26. doi: 10.1093/nar/10.15.4813.
8
The nonequilibrium character of DNA melting: effects of the heating rate on the fine structure of melting curves.DNA解链的非平衡特性：加热速率对解链曲线精细结构的影响
Nucleic Acids Res. 1984 May 25;12(10):4339-49. doi: 10.1093/nar/12.10.4339.
9
DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.通过单碱基对替换而不同的DNA片段在变性梯度凝胶中被分离：与解链理论的对应关系。
Proc Natl Acad Sci U S A. 1983 Mar;80(6):1579-83. doi: 10.1073/pnas.80.6.1579.
10
The effect of nucleotide substitution on DNA denaturation profiles.核苷酸取代对DNA变性图谱的影响。
Nucleic Acids Res. 1987 Aug 25;15(16):6665-76. doi: 10.1093/nar/15.16.6665.

本文引用的文献

1
DNA sequencing and helix-coil transition. III. DNA sequencing.DNA测序与螺旋-线圈转变。III. DNA测序。
Biopolymers. 1980 Jan;19(1):95-109. doi: 10.1002/bip.1980.360190107.
2
A study of the reversibility of helix-coil transition in DNA.一项关于DNA中螺旋-线圈转变可逆性的研究。
Nucleic Acids Res. 1981 Aug 25;9(16):4043-59. doi: 10.1093/nar/9.16.4043.
3
Fine structure in the thermal denaturation of DNA: high temperature-resolution spectrophotometric studies.DNA热变性的精细结构：高温分辨率分光光度研究
CRC Crit Rev Biochem. 1980;9(2):87-144. doi: 10.3109/10409238009105432.
4
Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization.质粒ColE1 DNA的平衡熔解：电子显微镜观察
Nucleic Acids Res. 1980 Sep 25;8(18):4165-84. doi: 10.1093/nar/8.18.4165.
5
Simplification of the empirical relationship between melting temperature of DNA, its GC content and concentration of sodium ions in solution.DNA 熔解温度、其 GC 含量与溶液中钠离子浓度之间经验关系的简化。
Biopolymers. 1971;10(12):2623-4. doi: 10.1002/bip.360101223.
6
Salt effects on the denaturation of DNA. 3. A calorimetric investigation of the transition enthalpy of calf thymus DNA in Na2SO4 solutions of varying ionic strength.盐对DNA变性的影响。3. 不同离子强度的Na2SO4溶液中小牛胸腺DNA转变焓的量热研究。
Biochim Biophys Acta. 1974 Feb 27;340(1):16-30. doi: 10.1016/0005-2787(74)90170-1.
7
Helix coil transitions of d(A)n-d(T)n, d(A-T)n-d(A-T)n, and d(A-A-T)n-d(A-T-T)n; evaluation of parameters governing DNA stability.
Biopolymers. 1977 May;16(5):1115-37. doi: 10.1002/bip.1977.360160512.
8
Theory of DNA melting curves.DNA熔解曲线理论
Biopolymers. 1977 Dec;16(12):2693-704. doi: 10.1002/bip.1977.360161209.
9
Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.小ColE1衍生物的核苷酸序列：自主复制和大肠杆菌素E1免疫所需区域的结构
Mol Gen Genet. 1979 May 4;172(2):151-9. doi: 10.1007/BF00268276.
10
DNA sequencing and melting curve.DNA测序与熔解曲线。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):101-5. doi: 10.1073/pnas.76.1.101.

DNA协同因子的测定

Determination of DNA cooperativity factor.

作者信息

Amirikyan B R, Vologodskii A V

出版信息

Nucleic Acids Res. 1981 Oct 24;9(20):5469-82. doi: 10.1093/nar/9.20.5469.

DOI:10.1093/nar/9.20.5469
PMID:7029470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327533/
Abstract

The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

摘要

本文介绍了对colE1 DNA区域位于DNA螺旋内部及其末端时熔点差异的测量。基于螺旋-线圈转变严格理论的计算与0.2M Na+（完全可逆熔解条件）下的实验数据直接比较，得到协同因子σ的值为2.5 - 5×10⁻⁵。我们讨论了DNA熔解的可逆性以及将“全或无”概念应用于DNA区域熔解的可能性。