Monath T P, Cropp C B, Muth D J, Calisher C H
Trans R Soc Trop Med Hyg. 1981;75(2):282-6. doi: 10.1016/0035-9203(81)90335-7.
The indirect fluorescent antibody (FA) test was evaluated for the serodiagnosis of yellow fever (YF) cases and for detection of antibodies after 17D YF vaccination. Results were compared with those of standard serological tests, reactions with heterologous flaviviral antigens were determined, and both IgM and IgG antibodies were measured. Spot slides of infected Vero cells were used as antigen substrate. In cases of primary YF infection, the FA test for IgG antibodies provided clear diagnostic results. Indirect FA tests with anti-IgG were more sensitive than the complement-fixation (CF) test and showed specificity comparable to the CF and neutralization (N) test. Cases of YF infection superimposed upon pre-existing heterologous flaviviral immunity developed broadly-crossreactive IgG antibodies. IgM antibodies were highly specific in cases of both primary infection and superinfection but were not consistently present. Most individuals without previous flaviviral exposure who received 17D vaccine failed to develop detectable antibodies by the indirect FA test; of those with pre-existing immunity, 79% developed IgG antibodies. The indirect FA test provides a simple, rapid diagnostic procedure which should be especially useful in one-site epidemiological investigations of YF outbreaks.
对间接荧光抗体（FA）试验进行了评估，以用于黄热病（YF）病例的血清学诊断以及17D黄热病疫苗接种后抗体的检测。将结果与标准血清学试验的结果进行比较，确定与异源黄病毒抗原的反应，并同时检测IgM和IgG抗体。使用感染的Vero细胞斑点玻片作为抗原底物。在原发性黄热病感染病例中，针对IgG抗体的FA试验提供了明确的诊断结果。用抗IgG进行的间接FA试验比补体结合（CF）试验更敏感，并且显示出与CF试验和中和（N）试验相当的特异性。在先前存在的异源黄病毒免疫基础上叠加的黄热病感染病例产生了广泛交叉反应的IgG抗体。IgM抗体在原发性感染和重叠感染病例中具有高度特异性，但并非始终存在。大多数未曾接触过黄病毒的个体接种17D疫苗后，通过间接FA试验未能检测到抗体；在那些先前具有免疫力的个体中，79%产生了IgG抗体。间接FA试验提供了一种简单、快速的诊断方法，在黄热病疫情的现场流行病学调查中应特别有用。
