通过高浓度氯化钠处理对核糖体亚基进行部分脱蛋白：蛋白质缺失颗粒的物理和化学特性

Partial deproteinization of ribosomal subunits by treatment with high concentrations of sodium chloride: physical and chemical characterization of protein depleted particles.

作者信息

Guerin M F, Hayes D H, Favre A

出版信息

Biochimie. 1981 Aug-Sep;63(8-9):685-97. doi: 10.1016/s0300-9084(81)80217-9.

Abstract

Sedimentation of E. coli ribosomal subunits through sucrose gradients containing high concentrations of NaCl (0.1 - 1.0 M) brings about removal of a specific fraction of their proteins. The alterations in the structural properties of the subunits caused by dissociation of these proteins are studied. They appear to be, at least partly the result of removal of constraints on RNA conformation imposed by the released proteins.

摘要

通过含有高浓度氯化钠（0.1 - 1.0 M）的蔗糖梯度对大肠杆菌核糖体亚基进行沉降，会导致其特定部分蛋白质的去除。研究了这些蛋白质解离所引起的亚基结构特性的改变。它们似乎至少部分是由于去除了释放的蛋白质对RNA构象施加的限制所致。