N-2-芴基乙酰胺诱导的大鼠肝癌细胞胞质溶胶中一种中性蛋白酶的活性增加。
Increased activity of a neutral protease in cytosol from rat hepatoma induced by N-2-fluorenylacetamide.
作者信息
Wada K, Matsui H, Tsukada K
出版信息
Cancer Res. 1981 Dec;41(12 Pt 1):5130-3.
A protease active with N-alpha-benzoyl-DL-arginine-p-nitroanilide with an optimum pH of 7.3 has been found in the cytosol of rat liver. The activity of this protease increased in N-2-fluorenylacetamide-induced hepatoma as well as in fetal liver. It has been purified from normal liver and hepatoma about 200-fold. Its molecular weight is estimated by gel filtration to be about 200,000 in each tissue. The protease activity is unaffected by chymostatin, pepstatin, soybean trypsin inhibitor, and p-chloromercuribenzoate. Antipain, leupeptin, tosyl-L-lysine chloromethyl ketone, and phenylmethylsulfonyl fluoride inhibit the protease activity. This protease appears to be a serine protease.
在大鼠肝脏的细胞溶质中发现了一种对N-α-苯甲酰-DL-精氨酸-对硝基苯胺有活性、最适pH为7.3的蛋白酶。这种蛋白酶的活性在N-2-芴基乙酰胺诱导的肝癌以及胎儿肝脏中有所增加。它已从正常肝脏和肝癌中纯化了约200倍。通过凝胶过滤估计其在每个组织中的分子量约为200,000。该蛋白酶活性不受抑糜酶素、胃蛋白酶抑制剂、大豆胰蛋白酶抑制剂和对氯汞苯甲酸的影响。抗蛋白酶、亮抑酶肽、甲苯磺酰-L-赖氨酸氯甲基酮和苯甲基磺酰氟可抑制该蛋白酶活性。这种蛋白酶似乎是一种丝氨酸蛋白酶。
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