Chino F
Acta Pathol Jpn. 1981 Nov;31(6):963-72. doi: 10.1111/j.1440-1827.1981.tb02010.x.
Brain material used in this experiment was from a 47-year-old woman autopsied 5 hours after death. The brain was markedly atrophic weighing 750 g. Histopathologically, a spongy state was extensive from cortex to medulla with fibrous astrocytosis and neuronal loss. This brain tissue was aseptically removed at autopsy and a 20% emulsion was prepared. Intracranially 0.1 ml and intraperitoneally 0.2 ml of the emulsion were inoculated into each guinea pig. Five and three of them were killed 10 and 22 months later, respectively, nevertheless showing no clinical signs. The brains from these guinea pigs did not reveal any changes as seen in the human autopsy brain both macroscopically and microscopically. However, all the brains indicated ultrastructural changes characterized by vacuolation with curled membranes in dendrites and free membranes with cellular debris in extracellular space. These changes were not found in guinea pigs inoculated with material from normal guinea pig brain or uninoculated guinea pigs, but similar to those observed in the human autopsy brain used as inoculum. Therefore, it could be considered that Creutzfeldt-Jakob disease was transmitted from man to guinea pig to present an early stage of the lesion. Several organs were observed ultrastructurally after whole bleeding in monkeys. Curled membranes were generated only on the vacuoles of dendrite from 2 1/2 hours after bleeding and not in those of other organs. This may explain at least a part of the pathogenesis of spongiform changes restricted in the central nervous system.
本实验所用的脑材料取自一名47岁女性，其在死后5小时进行了尸检。大脑明显萎缩，重750克。组织病理学检查显示，从皮质到髓质广泛存在海绵状状态，伴有纤维性星形胶质细胞增生和神经元丢失。在尸检时无菌取出该脑组织并制备成20%的乳剂。将0.1毫升乳剂颅内接种到每只豚鼠体内，0.2毫升乳剂腹腔内接种。分别在10个月和22个月后处死其中5只和3只豚鼠，然而它们均未表现出任何临床症状。这些豚鼠的大脑在宏观和微观上均未显示出人类尸检大脑中所见的任何变化。然而，所有大脑均显示出超微结构变化，其特征为树突中出现有空泡化且伴有卷曲膜，细胞外空间有游离膜和细胞碎片。在接种正常豚鼠脑材料的豚鼠或未接种的豚鼠中未发现这些变化，但与用作接种物的人类尸检大脑中观察到的变化相似。因此，可以认为克雅氏病从人传播给豚鼠后呈现出病变的早期阶段。对猴子全血后几个器官进行了超微结构观察。出血后2个半小时，仅在树突的空泡上产生卷曲膜，其他器官则未出现。这至少可以解释中枢神经系统中海绵状变化发病机制的一部分。
