Whitfield J F, Boynton A L, Healy G M, Sun A M
Cancer Lett. 1981 Nov;14(2):159-68. doi: 10.1016/0304-3835(81)90126-9.
Islet cells were isolated from 2 fetal bovine pancreas glands and cultivated in vitro. During the course of repeated passage in vitro, the B-cells in these cultures retained the ability to synthesize insulin, but rapidly lost the ability to secrete it. The cells also became progressively more able to proliferate in calcium-deficient medium which did not support the proliferation of cells from primary cultures. The reductions of insulin secretion and proliferative calcium dependence were accompanied by the acquisition of the ability to produce tumors in nude mice.
从2个胎牛胰腺中分离出胰岛细胞并进行体外培养。在体外反复传代过程中，这些培养物中的B细胞保留了合成胰岛素的能力，但迅速丧失了分泌胰岛素的能力。这些细胞在不支持原代培养细胞增殖的缺钙培养基中也逐渐变得更易于增殖。胰岛素分泌减少和增殖对钙的依赖性降低伴随着在裸鼠中产生肿瘤能力的获得。
