Swierenga S H, Whitfield J F, Gillan D J
J Natl Cancer Inst. 1976 Jul;57(1):125-9. doi: 10.1093/jnci/57.1.125.
Cells from the thigh muscles of normal fetal rats proliferated rapidly and indefinitely in a medium containing adult rat "plasma" and a normal free-calcium concentration, but they could not proliferate in calcium-deficient plasma medium. As the animals grew older, the cells became increasingly less able to proliferate even in normal (high-calcium) plasma medium, though they retained the potential to proliferate in a more conventional medium containing fetal bovine serum. By contrast, neoplastic adult cells from malignant rhabdomyosarcomas (induced by Ni3S2) proliferated rapidly and indefinitely in both normal and low-calcium plasma medium
正常胎鼠大腿肌肉细胞在含有成年大鼠“血浆”和正常游离钙浓度的培养基中迅速且无限增殖，但在缺钙血浆培养基中无法增殖。随着动物年龄增长，即使在正常（高钙）血浆培养基中，细胞增殖能力也越来越弱，不过它们在含有胎牛血清的更传统培养基中仍保留增殖潜力。相比之下，来自恶性横纹肌肉瘤（由Ni3S2诱导）的成年肿瘤细胞在正常和低钙血浆培养基中均能迅速且无限增殖。
