延迟添加抗原和“E”花环形成对正常个体、结核病患者或麻风病患者外周血淋巴细胞对分枝杆菌抗原增殖反应的影响。
The effect of delayed addition of antigen and 'E' rosetting on the proliferative response to mycobacterial antigens of peripheral blood lymphocytes from normal individuals or from patients with tuberculosis or leprosy.
作者信息
Bahr G M, Rook G A, Stanford J L, Lydyard P M, Bryceson A D
出版信息
Immunology. 1981 Nov;44(3):585-91.
Some suppressor cells are reported to lose their activity when precultured without stimulus in vitro. We have investigated the role of such suppressors in responsiveness to mycobacterial antigens of peripheral blood mononuclear cells (PBMNC) from patients with leprosy or tuberculosis, or from normal donors. Delayed addition of mycobacterial antigens (Mycobacterium leprae, Mycobacterium vaccae and Mycobacterium tuberculosis), but not of a fungal antigen (Candida albicans) caused enhanced responses using PBMNC from most normal donors, or tuberculoid leprosy (TT/BT) patients. However, the effect was less common using PBMNC from the lepromatous leprosy (BL/LL) group. (P less than 01.01, using M. leprae, relative to the TT/BT group), suggesting that this type of suppression reflects a normal mechanism, which is diminished rather than increased in anergic patients. Delayed addition of antigens to 'E'-rosetting cells did not result in enhanced responses. However, the different effects of 'E'-rosetting on the responses to the mycobacterial antigens of cells from normals, TT/BT and BL/LL patients, suggested that there may be two types of proliferative response to these antigens.
据报道，一些抑制细胞在体外无刺激预培养时会失去活性。我们研究了此类抑制细胞在麻风病或结核病患者或正常供体外周血单个核细胞（PBMNC）对分枝杆菌抗原反应中的作用。延迟添加分枝杆菌抗原（麻风分枝杆菌、母牛分枝杆菌和结核分枝杆菌），而非真菌抗原（白色念珠菌），可增强大多数正常供体或结核样麻风（TT/BT）患者的PBMNC的反应。然而，使用瘤型麻风（BL/LL）组的PBMNC时，这种效应不太常见。（相对于TT/BT组，使用麻风分枝杆菌时P<0.01），这表明这种类型的抑制反映了一种正常机制，在无反应性患者中这种机制减弱而非增强。向“E”花环细胞延迟添加抗原并未导致反应增强。然而，“E”花环对正常、TT/BT和BL/LL患者细胞对分枝杆菌抗原反应的不同影响表明，对这些抗原可能存在两种增殖反应类型。
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