Deetz L E, Wangsness P J
J Anim Sci. 1981 Aug;53(2):427-33. doi: 10.2527/jas1981.532427x.
The effect of intrajugular injections of insulin, glucagon and propionate, administered singly and in combination, as possible peripheral feedbacks in the control of feed intake in wethers was studied. A complete mixed diet (25% chopped hay: 75% cracked corn) was fed ad libitum. The treatments were saline, 6 mU insulin/kg body weight (BW), 9 ng glucagon/kg BW and 1.3 mg propionate/kg BW. In Exp. 1, five wethers were given the treatments at the beginning of each spontaneous meal over a 24-hr period, and total daily feed intakes were measured. The average number of injections per sheep for a 24-hr period was eight. In Exp. 2, the effects of the treatments on plasma concentrations of insulin, glucagon, propionate and glucose at 15, 30, 60 and 120 min after injection were measured in six other wethers. In Exp. 1, insulin (P less than .01), glucagon (P less than .01), insulin plus propionate (P less than .05) and glucagon plus propionate (P less than .05) decreased 24-hr feed intake by 18.5, 15.8, 11.0 and 11.8%, respectively, compared to the saline control. In Exp. 2, plasma insulin concentrations were increased (P less than .05) at 15 min after administration of insulin and insulin plus propionate, to 2.0 and 2.1 times the preinjection levels, respectively. Glucagon concentrations in plasma were increased (P less than .01) at 15 min after the injection of glucagon, to 2.0 times the pretreatment values. Insulin and glucagon concentrations in plasma were increased only slightly (P less than .10) after administration of glucagon plus propionate. No treatments affected glucose or propionate concentrations in the plasma. Increases in plasma concentrations of insulin, glucagon and propionate may interact directly or initiate other mechanisms involved in the short-term control of feed intake by sheep on a concentrate diet.
研究了颈静脉注射胰岛素、胰高血糖素和丙酸盐单独及联合给药作为可能的外周反馈对去势公羊采食量控制的影响。自由采食一种完全混合日粮（25%切碎干草：75%破碎玉米）。处理组分别为生理盐水、6 mU胰岛素/千克体重、9 ng胰高血糖素/千克体重和1.3 mg丙酸盐/千克体重。在实验1中，5只去势公羊在24小时内每次自发进食开始时接受处理，并测量每日总采食量。每只羊在24小时内的平均注射次数为8次。在实验2中，在另外6只去势公羊中测量了注射后15、30、60和120分钟时处理对胰岛素、胰高血糖素、丙酸盐和葡萄糖血浆浓度的影响。在实验1中，与生理盐水对照组相比，胰岛素（P＜0.01）、胰高血糖素（P＜0.01）、胰岛素加丙酸盐（P＜0.05）和胰高血糖素加丙酸盐（P＜0.05）使24小时采食量分别降低了18.5%、15.8%、11.0%和11.8%。在实验2中，注射胰岛素和胰岛素加丙酸盐后15分钟时血浆胰岛素浓度升高（P＜0.05），分别升至注射前水平的2.0倍和2.1倍。注射胰高血糖素后15分钟时血浆胰高血糖素浓度升高（P＜0.01），升至预处理值的2.0倍。注射胰高血糖素加丙酸盐后，血浆中胰岛素和胰高血糖素浓度仅略有升高（P＜0.10）。没有处理影响血浆中的葡萄糖或丙酸盐浓度。血浆中胰岛素、胰高血糖素和丙酸盐浓度的升高可能直接相互作用，或启动其他参与短期控制采食精料日粮的绵羊采食量的机制。
