具有互补互补位和独特型位的免疫球蛋白。
Immunoglobulin with complementary paratope and idiotope.
作者信息
Kang C Y, Kohler H
出版信息
J Exp Med. 1986 Apr 1;163(4):787-96. doi: 10.1084/jem.163.4.787.
Abstract
A hybridoma antibody (11E7-1) was isolated from a myeloma fusion with nu/nu BALB/c immunized against the T15 idiotype. This IgM antibody exhibited a dual specificity, binding both to PC and to anti-PC antibodies from two idiotype families. Binding to PC and anti-PC antibodies are completely inhibited by PC analogs. Furthermore, the hybridoma antibody binds to itself. Self-binding is also inhibited by PC analogs. From these data, we suggest that 11E7-1 hybridoma antibody has a PC-specific paratope site, and at same time expresses the internal PC antigen idiotope. The term autobody is proposed to signify its self-binding and potential role in autoimmunity. Autobodies may have a unique role in the network of immune system. Furthermore, it may be a model for designing idiotype vaccines.
摘要
从与针对T15独特型免疫的裸鼠/裸BALB/c骨髓瘤融合物中分离出一种杂交瘤抗体（11E7-1）。这种IgM抗体表现出双重特异性，既能与磷脂酰胆碱（PC）结合，也能与来自两个独特型家族的抗PC抗体结合。与PC和抗PC抗体的结合完全被PC类似物抑制。此外，杂交瘤抗体能与自身结合。自身结合也被PC类似物抑制。根据这些数据，我们认为11E7-1杂交瘤抗体具有PC特异性互补决定区位点，同时表达内部PC抗原独特型。提出“自身抗体”一词来表示其自身结合以及在自身免疫中的潜在作用。自身抗体可能在免疫系统网络中具有独特作用。此外，它可能是设计独特型疫苗的一个模型。
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