Veilleux B G, Rowland I
J Gen Microbiol. 1981 Mar;123(1):103-15. doi: 10.1099/00221287-123-1-103.
To investigate the ecological mechanisms governing the community structure of the gut microbial ecosystem, we have attempted to simulate the rat gut ecosystem in vitro using a two-stage continuous culture. Extensive sampling of the rat hindgut has established a set of criteria with which the in vitro system may be compared. This paper discusses one of the criteria, the community composition and structure in vivo and in vitro. The experiments indicated that a gut microbial ecosystem could be satisfactorily mimicked in vitro. This was achieved using a two-stage continuous culture employing differential selection of species between the two stages on the basis of pH differences combined with cell recycling between stages.
为了研究控制肠道微生物生态系统群落结构的生态机制，我们尝试使用两阶段连续培养在体外模拟大鼠肠道生态系统。对大鼠后肠进行的广泛采样建立了一套可用于与体外系统进行比较的标准。本文讨论其中一项标准，即体内和体外的群落组成与结构。实验表明，体外能够令人满意地模拟肠道微生物生态系统。这是通过两阶段连续培养实现的，该培养基于pH差异在两个阶段之间进行物种的差异选择，并结合阶段间的细胞循环。
