Molly K, Vande Woestyne M, Verstraete W
Laboratory of Microbial Ecology, Faculty of Agricultural and Applied Biological Sciences, University of Gent, Belgium.
Appl Microbiol Biotechnol. 1993 May;39(2):254-8. doi: 10.1007/BF00228615.
A five-stage reactor was developed to simulate the gastro-intestinal microbial ecosystem of humans. The small intestine was simulated by a two-step "fill and draw" system, the large intestine by a three-step reactor. A representative supply medium was developed to support a microbial community resembling that of the human gastro-intestinal tract. The entire system was validated by monitoring fermentation fluxes and products, i.e. indicator bacterial groups, volatile fatty acids, enzymatic activities and headspace gases. The simulator was operated with varying concentrations and combinations of arabinogalactan, xylan, pectin, dextrins and starch. The resulting patterns of microbial diversity and activity were analysed and compared with data for in-vivo gastro-intestinal microbial communities as described in the literature and found to be representative.
开发了一种五阶段反应器来模拟人类胃肠道微生物生态系统。小肠由两步“填充和抽取”系统模拟，大肠由三步反应器模拟。开发了一种代表性的供应培养基，以支持类似于人类胃肠道的微生物群落。通过监测发酵通量和产物，即指示细菌群、挥发性脂肪酸、酶活性和顶空气体，对整个系统进行了验证。模拟器在不同浓度和组合的阿拉伯半乳聚糖、木聚糖、果胶、糊精和淀粉下运行。分析了由此产生的微生物多样性和活性模式，并与文献中描述的体内胃肠道微生物群落数据进行了比较，发现具有代表性。
