Derouette S, Hornebeck W, Loisance D, Godeau G, Cachera J P, Robert L
Pathol Biol (Paris). 1981 Nov;29(9):539-47.
Insoluble elastins were isolated from control and aneurytic aortas by a sequential extraction procedure involving the use of purified collagenase. Marked differences in amino acid analyses and susceptibilities to pancreatic elastase were observed between normal and pathological samples. The incorporation of either 14C-lysine or 14C-glucosamine into proteins of the vessel wall was also studied. In addition, high amounts of elastase-type activity was extractable from pathological aorta specimens which may contribute significantly to the loss of elastic tissue evidenced by ultra-structural studies and confirmed by the biochemical technics. We propose therefore that increased elastase-type protease activity in these pathological aortas does significantly contribute to the weakening of the aortic wall and also may well be the main cause of the rupture of aneurysms observed occasionally.
通过使用纯化的胶原酶的顺序提取程序，从对照和动脉瘤主动脉中分离出不溶性弹性蛋白。在正常和病理样本之间观察到氨基酸分析和对胰弹性蛋白酶敏感性的显著差异。还研究了将14C-赖氨酸或14C-葡萄糖胺掺入血管壁蛋白质中的情况。此外，从病理主动脉标本中可提取出大量弹性酶型活性，这可能对超微结构研究所证明并经生化技术证实的弹性组织损失有显著贡献。因此，我们提出，这些病理主动脉中弹性酶型蛋白酶活性的增加确实对主动脉壁的削弱有显著贡献，并且很可能也是偶尔观察到的动脉瘤破裂的主要原因。
