Melico-Silvestre A A, Jacotot B, Buxtorf J C, Beaumont V, Beaumont J L
Pathol Biol (Paris). 1981 Nov;29(9):573-8.
Free and esterified cholesterol in the skin were assayed by gas chromatography after thin layer histochromatographic separation. There were 20 controls of both sexes, 36 subjects with type lla hyperlipoproteinemia, 27 with type IIb hyperlipoproteinemia and 19 with type IV hyperlipoproteinemia. Esterified skin cholesterol levels were higher in women with the type of lla disorder and in subjects with tendinous xanthomas. The levels of esterified skin cholesterol in type II women is not correlated with free skin cholesterol or with total circulating cholesterol; all these parameters are correlated in men. Furthermore, skin cholesterol levels were determined in 15 type II subjects before and after a six month treatment with the anticholesterolemic probucol. Cholesterolemia decreased 10 per cent, free skin cholesterol decreased slightly and esterified skin cholesterol increased significantly, as did the esterified/total skin cholesterol ratio. These data suggest the existence of a relationship between low vascular risk and elevated levels of esterified skin cholesterol (or elevated esterified/total skin cholesterol ratio).
皮肤中游离胆固醇和酯化胆固醇经薄层组织色谱分离后，采用气相色谱法进行测定。有20名男女对照者，36名IIa型高脂蛋白血症患者，27名IIb型高脂蛋白血症患者和19名IV型高脂蛋白血症患者。IIa型疾病女性患者和有腱性黄色瘤的患者皮肤酯化胆固醇水平较高。II型女性患者皮肤酯化胆固醇水平与皮肤游离胆固醇或总循环胆固醇均无相关性；在男性中，所有这些参数均具有相关性。此外，对15名II型受试者在使用抗胆固醇药物普罗布考进行为期6个月的治疗前后测定了皮肤胆固醇水平。血胆固醇水平下降了10%，皮肤游离胆固醇略有下降，皮肤酯化胆固醇显著增加，皮肤酯化/总胆固醇比值也增加。这些数据表明低血管风险与皮肤酯化胆固醇水平升高（或酯化/总皮肤胆固醇比值升高）之间存在关联。
