Bor N M, Alvur M, Ercan M T, Bekdik C F
Res Exp Med (Berl). 1981;179(3):261-8. doi: 10.1007/BF01851624.
Dogs in postabsorbtive state were anesthetized with IV nembutal. Their femoral arteries were catheterized, and the abdomens were entered via a midline incision. A small branch of the pancreatic artery and a corresponding small vein were catheterized. 133Xe was injected through the artery, and the the pancreatic blood flow rate (PBFR) was determined. Net insulin secretion was calculated using the insulin concentration of pancreatic vein-artery difference and pancreatic plasma flow rate (PPFR). After control studies an oral glucose tolerance test (2 g/kg body wt.) was performed. Under control studies the mean value of net insulin secretion was 3,753.2 +/- 699.1 microunits/100 g P/min and the maximum values were 10,610.2 +/- 3,658.7 and 11,108.0 +/- 2,852.6 microunits/100 g P/min at 20 and 60 min after glucose loading, respectively (P less than 0.05). Insulin response per mg of glucose was 83.2 +/- 12.2 microunits under control conditions. Twenty minutes after glucose loading this figure rose to 173.4 +/- 41.7 microunits/mg glucose and at 60 min 207.9 +/- 49.2 microunits/mg glucose.
处于吸收后状态的狗通过静脉注射戊巴比妥钠进行麻醉。将导管插入它们的股动脉，经中线切口打开腹腔。将胰动脉的一个小分支和相应的一条小静脉插入导管。通过动脉注射133Xe，并测定胰腺血流速率（PBFR）。使用胰腺静脉-动脉差的胰岛素浓度和胰腺血浆流速（PPFR）计算净胰岛素分泌量。在对照研究后，进行口服葡萄糖耐量试验（2 g/kg体重）。在对照研究中，净胰岛素分泌的平均值为3,753.2±699.1微单位/100 g胰腺/分钟，在葡萄糖负荷后20分钟和60分钟时的最大值分别为10,610.2±3,658.7和11,108.0±2,852.6微单位/100 g胰腺/分钟（P<0.05）。在对照条件下，每毫克葡萄糖的胰岛素反应为83.2±12.2微单位。葡萄糖负荷后20分钟，该数值升至173.4±41.7微单位/毫克葡萄糖，60分钟时为207.9±49.2微单位/毫克葡萄糖。
