培养厌氧菌的简单便捷方法。

Simple and convenient method for culturing anaerobic bacteria.

作者信息

Behbehani M J, Jordan H V, Santoro D L

出版信息

Appl Environ Microbiol. 1982 Jan;43(1):255-6. doi: 10.1128/aem.43.1.255-256.1982.

DOI:10.1128/aem.43.1.255-256.1982

PMID:7034646

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241812/

Abstract

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic incubation of broth cultures in Brewer Anaerobic Jars.

摘要

本文描述了一种简单便捷的厌氧菌培养方法。培养物可在通常用于制备无菌外用溶液的市售烧瓶中生长。一种特殊的一次性橡胶烧瓶密封盖在高压灭菌后可维持烧瓶内的厌氧条件。多种口腔厌氧菌的生长情况与在布鲁尔厌氧罐中对肉汤培养物进行厌氧培养后的生长情况相当。