固体培养基中厌氧菌的生长曲线。
Growth curves of anaerobic bacteria in solid media.
作者信息
Casciato D A, Stewart P R, Rosenblatt J E
出版信息
Appl Microbiol. 1975 May;29(5):610-4. doi: 10.1128/am.29.5.610-614.1975.
Abstract
Simple pour plate and spectrophotometric techniques for the evaluation of growth curves of several anaerobic bacteria on solid media are described. Three basic patterns of anaerobic growth were observed. The curves obtained were very reproducible when studied on separate occasions. The curves obtained by spectrophotometric measurement were comparable to those obtained by the pour plate method, especially when a large bacterial inoculum was used. Limitations in the interpretation of the results are discussed. The methods and principles reported could provide the basis for the determination of bacterial growth on solid media using other organisms and different experimental conditions.
摘要
描述了用于评估几种厌氧菌在固体培养基上生长曲线的简单倾注平板法和分光光度法。观察到三种基本的厌氧生长模式。在不同时间进行研究时，所获得的曲线具有很高的重现性。通过分光光度法测量获得的曲线与倾注平板法获得的曲线相当，尤其是在使用大量细菌接种物时。讨论了结果解释中的局限性。所报道的方法和原理可为使用其他生物体和不同实验条件测定固体培养基上的细菌生长提供基础。
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