Kroll R G, Booth I R
Biochem J. 1981 Sep 15;198(3):691-8. doi: 10.1042/bj1980691.
The role of K+ transport in the generation of a pH gradient in Escherichia coli has been investigated. In K+-depleted cells, net K+ uptake dissipated delta psi (membrane potential) and led to an increase in delta pH (pH gradient). The magnitude of the delta pH formed bore a simple relationship to the net K+ uptake and was substantially independent of the respiratory rate. In K+-replete cells, generation of a pH gradient was again K+-dependent, although no net uptake of this cation occurred. The results are discussed in terms of K+ cycling, and it is suggested that delta pH is in part a function of the rate of cycling and independent of the respiratory rate.
对钾离子转运在大肠杆菌pH梯度形成过程中的作用进行了研究。在钾离子缺乏的细胞中，钾离子的净摄取消耗了膜电位（Δψ），并导致pH梯度（ΔpH）增加。形成的ΔpH大小与钾离子的净摄取呈简单关系，且基本独立于呼吸速率。在钾离子充足的细胞中，pH梯度的形成同样依赖于钾离子，尽管该阳离子没有发生净摄取。根据钾离子循环对结果进行了讨论，并表明ΔpH部分是循环速率的函数，且独立于呼吸速率。
