通过细胞膜的能量化作用控制大肠杆菌中磷酸烯醇丙酮酸依赖性磷酸转移酶介导的糖转运
Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.
作者信息
Reider E, Wagner E F, Schweiger M
出版信息
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5529-33. doi: 10.1073/pnas.76.11.5529.
Abstract
The phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli is inhibited by the energized of the membrane. This was shown in intact cells as well as in membrane vesicles. Relaxation of the proton gradient by uncouplers stimulated the uptake of sugars via the phosphotransferase system in aerobically cultured cells. No such effect was seen in anaerobic cells, apparently because the cell membrane of these cells is poorly energized. Energization by respiration of D-lactate or ascorbate inhibited the phosphotransferase uptake system in membrane vesicles. This inhibition was reversed by the addition of cyanide. Oxamate, a specific inhibitor of lactate dehydrogenase, prevented the inhibitory effect of D-lactate. Membrane vesicles prepared from a cytochrome-less mutant were not energized by D-lactate oxidation and the phosphotransferase uptake system was not inhibited.
摘要
大肠杆菌中磷酸烯醇丙酮酸依赖性磷酸转移酶介导的糖转运受到膜的能量化抑制。这在完整细胞以及膜囊泡中均得到证实。解偶联剂使质子梯度松弛，刺激了需氧培养细胞中通过磷酸转移酶系统的糖摄取。在厌氧细胞中未观察到这种效应，显然是因为这些细胞的细胞膜能量化程度较差。D-乳酸或抗坏血酸呼吸产生的能量化抑制了膜囊泡中的磷酸转移酶摄取系统。添加氰化物可逆转这种抑制作用。乳酸脱氢酶的特异性抑制剂草氨酸盐可防止D-乳酸的抑制作用。从无细胞色素突变体制备的膜囊泡不会因D-乳酸氧化而能量化，磷酸转移酶摄取系统也不会受到抑制。
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Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.通过细胞膜的能量化作用控制大肠杆菌中磷酸烯醇丙酮酸依赖性磷酸转移酶介导的糖转运
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