低剂量全身照射后脾切除怀孕小鼠的造血作用

Hemopoiesis in the splenectomized-pregnant mouse following low-dose total-body irradiation.

作者信息

Weinberg S R, MacVittie T J

出版信息

Exp Hematol. 1981 Sep;9(8):849-55.

Abstract

The effect of splenectomy (SPLX) and total-body irradiation (TBI) (50-200 rad) on virgin and pregnant mouse hemopoiesis was studied, using peripheral blood hemogram values and femoral marrow hemopoietic progenitor cell activity (i.e., CFUE, BFUE, and GM-CFC). The SPLX-maternal red cell counts and hematocrit values were lower than those of SPLX-virgin mice, reflecting the anemia of pregnancy. But the white cell counts of both SPLX-virgin and SPLX-day-14.5 pregnant mice were significantly higher (P less than 0.005) than normal-virgin mice. Both nonirradiated and day-4 irradiated SPLX-maternal marrow Ep-independent and Ep-dependent CFUE were higher than the nonirradiated and day-4 irradiated SPLX-virgin values (respectively, for each TBI dose studied). On the other hand, nonirradiated and day-4 irradiated SPLX-maternal GM-CFC were lower than the nonirradiated and day-4 irradiated SPLX-virgin GM-CFC values. The data demonstrate the potential of the SPLX-maternal femoral marrow to respond to the stress of low-dose TBI with effective compensatory erythropoiesis, possibly at the expense of granulopoiesis.

摘要

利用外周血血细胞计数和股骨骨髓造血祖细胞活性（即CFUE、BFUE和GM-CFC），研究了脾切除术（SPLX）和全身照射（TBI）（50 - 200拉德）对未孕和怀孕小鼠造血功能的影响。脾切除的母体红细胞计数和血细胞比容值低于脾切除的未孕小鼠，反映出妊娠贫血。但脾切除的未孕小鼠和脾切除的妊娠14.5天小鼠的白细胞计数均显著高于正常未孕小鼠（P < 0.005）。对于所研究的每种TBI剂量，未照射和照射第4天的脾切除母体骨髓中不依赖促红细胞生成素（Ep）和依赖Ep的CFUE均高于未照射和照射第4天的脾切除未孕小鼠的值。另一方面，未照射和照射第4天的脾切除母体GM-CFC低于未照射和照射第4天的脾切除未孕小鼠的GM-CFC值。数据表明，脾切除的母体股骨骨髓有潜力通过有效的代偿性红细胞生成对低剂量TBI的应激作出反应，这可能以粒细胞生成受损为代价。