Ackermans F, Klein J P, Frank R M
J Biol Buccale. 1981 Sep;9(3):203-17.
The ultrastructural localization of bacterial antigens of Streptococcus sanguis and of the various serotypes a,b,c,d,e,f and g of Streptococcus mutans was studied in human carious dentine using the indirect peroxidase-antiperoxidase method with appropriate controls. No positive staining was seen in adjacent normal dentine. In the inner dentine underlying the cytoplasm of fibroblasts and Schwann cells of unmyelinated nerve fibrils. In sclerosed tubules, or on the plasmalemma of the odontoblast process or on both structures. Only in the odontoblast, facing the carious cone, were dense stainings noted in vacuoles of various sizes located in the Golgi apparatus in juxta-nuclear position and in their odontoblast processes. In pulpal regions, underlying the carious cone, dense vacuoles were also observed in the cytoplasm of fibroblasts and Schwann cells of unmyelinated nerve fibrils. In sclerosed tubules, electron dense deposits were noted in the lumen and the walls of the calcified tubules. In the outer carious dentine, invaded by micro-organisms, positive antigenic stainings were observed in the cell wall and capsular material of a great number of micro-organisms as well as in the interbacterial matrix.
采用间接过氧化物酶 - 抗过氧化物酶法并设置适当对照，研究了血链球菌细菌抗原以及变形链球菌a、b、c、d、e、f和g各血清型在人类龋损牙本质中的超微结构定位。在相邻的正常牙本质中未见阳性染色。在无髓神经纤维的成纤维细胞和施万细胞胞质下方的内层牙本质中、硬化小管内、成牙本质细胞突起的质膜上或在这两种结构上均未见阳性染色。仅在面向龋损锥的成牙本质细胞中，位于近核位置的高尔基体中各种大小的液泡及其成牙本质细胞突起中有密集染色。在龋损锥下方的牙髓区域，无髓神经纤维的成纤维细胞和施万细胞的胞质中也观察到密集的液泡。在硬化小管中，钙化小管的管腔和管壁中有电子致密沉积物。在被微生物侵入的外层龋损牙本质中，在大量微生物的细胞壁和荚膜物质以及细菌间基质中观察到阳性抗原染色。
