用于光镜和电镜观察的胶质纤维酸性蛋白包埋后免疫过氧化物酶染色。

Post-embedding immunoperoxidase staining of glial fibrillary acidic protein for light and electron microscopy.

作者信息

DeArmond S J, Siegel M W, Dixon R G, Eng L F

出版信息

J Neuroimmunol. 1981 Mar;1(1):3-15. doi: 10.1016/0165-5728(81)90003-5.

Abstract

Post-embedding peroxidase-antiperoxidase methods to stain glial fibrillary acidic (GFA) protein in Araldite-embedded sections for light and electron microscopy were developed. The Jimpy mouse spinal cord was used because it is gliotic and contains abundant glial filaments and GFA protein. For light microscopy, specific staining was obtained in thick and in ultrathin sections mounted on glass following removal of the plastic with sodium ethoxide. Consistent specific staining for GFA protein in ultrathin sections mounted on nickel grids required partial removal of the plastic with 1% sodium ethoxide and further treatment with 2% sodium dodecyl sulfate (SDS).

摘要

已开发出包埋后过氧化物酶-抗过氧化物酶方法，用于对环氧树脂包埋切片中的胶质纤维酸性（GFA）蛋白进行染色，以用于光学显微镜和电子显微镜观察。使用Jimpy小鼠脊髓，因为它发生了胶质化，含有丰富的胶质丝和GFA蛋白。对于光学显微镜观察，在用乙醇钠去除塑料后，安装在玻璃上的厚切片和超薄切片均获得了特异性染色。安装在镍网上的超薄切片中GFA蛋白的一致特异性染色需要用1%乙醇钠部分去除塑料，并用2%十二烷基硫酸钠（SDS）进一步处理。