Tager J M, Wanders R J, Groen A K, van der Meer R, Akerboom T P, Meijer A J
Acta Biol Med Ger. 1981;40(7-8):895-906.
The control of respiration was studied in isolated rat-liver cells and isolated rat-liver mitochondria. When isolated rat-liver cells are perfused with different concentrations of lactate at a constant lactate/pyruvate ratio, the rate of mitochondrial respiration is a linear function of the affinity for the respiratory chain; the affinity term contains the intramitochondrial redox state and the extramitochondrial phosphate potential. However, when the cells are perfused with ammonia in addition to lactate (+ pyruvate), the stimulation of the oxygen uptake is not accompanied by an increase in the affinity. In order to obtain further insight into the relationship between oxygen uptake and intramitochondrial and extramitochondrial phosphate potential, experiments were also carried out with isolate rat-liver mitochondria incubated with an extramitochondrial ATP-utilizing system (glucose plus hexokinase) or an intramitochondrial ATP-utilizing system (citrulline synthesis). The results suggest that the rate of oxygen consumption is controlled by the intramitochondrial phosphate potential.
在分离的大鼠肝细胞和分离的大鼠肝线粒体中研究了呼吸控制。当以恒定的乳酸/丙酮酸比率用不同浓度的乳酸灌注分离的大鼠肝细胞时，线粒体呼吸速率是对呼吸链亲和力的线性函数；亲和力项包含线粒体内氧化还原状态和线粒体外磷酸盐电位。然而，当除了乳酸（+丙酮酸）之外还用氨灌注细胞时，氧气摄取的刺激并不伴随着亲和力的增加。为了进一步深入了解氧气摄取与线粒体内和线粒体外磷酸盐电位之间的关系，还对与线粒体外ATP利用系统（葡萄糖加己糖激酶）或线粒体内ATP利用系统（瓜氨酸合成）一起孵育的分离大鼠肝线粒体进行了实验。结果表明，氧气消耗速率受线粒体内磷酸盐电位控制。
