Weringer E J, Kelso J M, Tamai I Y, Arquilla E R
Acta Endocrinol (Copenh). 1982 Jan;99(1):101-8. doi: 10.1530/acta.0.0990101.
In the present study wound healing was examined in normal C57B1/6 male mice, diabetic mice, non-treated; and in diabetic mice treated with insulin. Small dermal wounds were made in the ears of the mice 40 h after the initial injection of insulin or vehicle alone. All animals were biopsied 8 h later. The wounds were examined by light and electron microscopy and wound components (capillaries, fibroblasts, PMN's oedema, collagen) were quantitated by lineal point analysis. The non-treated, diabetic mice demonstrated in inability to heal wounds when compared to controls; whereas, diabetic mice given a multidose insulin regimen demonstrated a response similar to controls. Insulin treatment of diabetic mice reduced the mean level of hyperglycaemia when compared to the non-treated diabetic mice. There was no detectable difference in the healing response with duration of diabetes in either the insulin-treated or non-treated diabetic mice. Although there was a mild reduction in hyperglycaemia, these data support the hypotheses that insulin is a necessary component of an adequate wound healing response.
在本研究中，我们检测了正常C57B1/6雄性小鼠、未经治疗的糖尿病小鼠以及接受胰岛素治疗的糖尿病小鼠的伤口愈合情况。在首次注射胰岛素或仅注射赋形剂40小时后，在小鼠耳部制造小的皮肤伤口。8小时后对所有动物进行活检。通过光学显微镜和电子显微镜检查伤口，并通过线性点分析对伤口成分（毛细血管、成纤维细胞、中性粒细胞、水肿、胶原蛋白）进行定量。与对照组相比，未经治疗的糖尿病小鼠表现出伤口无法愈合的情况；而接受多剂量胰岛素治疗方案的糖尿病小鼠表现出与对照组相似的反应。与未治疗的糖尿病小鼠相比，胰岛素治疗糖尿病小鼠降低了高血糖的平均水平。在胰岛素治疗或未治疗的糖尿病小鼠中，伤口愈合反应与糖尿病病程之间没有可检测到的差异。尽管高血糖有轻微降低，但这些数据支持胰岛素是充分伤口愈合反应的必要组成部分这一假设。
