大疱性类天疱疮与抗核抗体。氯化钠处理底物中基底膜荧光的显现。

Bullous pemphigoid and antinuclear antibodies. Unmasking of basement membrane fluorescence in sodium chloride-treated substrates.

作者信息

Danno K, Okamoto H, Miyauchi H, Imamura S

出版信息

Arch Dermatol. 1982 Jan;118(1):37-9. doi: 10.1001/archderm.118.1.37.

DOI:10.1001/archderm.118.1.37

Abstract

Immunoflourescent (IF) microscopic studies were done on a patient with bullous pemphigoid who had a high titer of antinuclear antibodies (ANAs). Direct IF microscopy showed deposition of lgG and C3 at the basement membrane zone (BMZ), while, by indirect IF microscopy, ANAs (but not circulating pemphigoid antibodies) were detected. The 2M sodium chloride (NaCl) solution-treated substrates, in which nuclear antigens were eluted, uncovered pemphigoid antibodies. Treatment of the substrates with NaCl solution unmasked BMZ fluorescence in another set of experiments in which a large amount of serum samples containing ANAs was added to serum samples containing only pemphigoid antibodies. These results suggest that pemphigoid antibodies are able to react to the BMZ in the presence of concomitant ANAs unless bind to the nuclear antigens.

摘要

对一名抗核抗体（ANA）滴度高的大疱性类天疱疮患者进行了免疫荧光（IF）显微镜研究。直接免疫荧光显微镜检查显示IgG和C3沉积于基底膜带（BMZ），而间接免疫荧光显微镜检查检测到ANA（而非循环类天疱疮抗体）。用2M氯化钠（NaCl）溶液处理的底物洗脱了核抗原，从而发现了类天疱疮抗体。在另一组实验中，向仅含类天疱疮抗体的血清样本中加入大量含ANA的血清样本，用NaCl溶液处理底物后揭示了BMZ荧光。这些结果表明，除非与核抗原结合，类天疱疮抗体在存在伴随ANA的情况下能够与BMZ发生反应。