Bone A J, Swenne I, Hellerström C
Diabete Metab. 1981 Dec;7(4):259-63.
The effects of prolonged culture of rat pancreatic islets at a high glucose concentration have been investigated. Isolated islets were cultured for either one day, one week or four weeks in 5.5 mM or 55 mM D-glucose or 5.5 mM D-glucose plus 49.5 mM L-glucose. At the end of the culture periods both the basal and stimulated rates of (pro) insulin and total protein synthesis were measured in short-term incubations and the islet insulin content was also determined. After prolonged culture (1-4 weeks) the insulin content of islets maintained at 5.5 mM D-glucose (with or without L-glucose) increased 2.5-4 times, while that of the high-glucose exposed islets remained essentially unchanged. The (pro) insulin biosynthetic response to an acute glucose challenge, after both one day and one week was less marked in the 55 mM than in the 5.5 mM glucose cultured islets. This may largely be due to the high basal rates of (pro) insulin biosynthesis observed in the high glucose cultured islets. At the end of the fourth culture week the rates of (pro) insulin biosynthesis were similar in all three experimental groups. The results indicate that prolonged in vitro exposure of islets to an abnormally high glucose concentration does not result in any specific injurious effects on the B-cells.
研究了大鼠胰岛在高葡萄糖浓度下长时间培养的影响。分离的胰岛在5.5 mM或55 mM D -葡萄糖或5.5 mM D -葡萄糖加49.5 mM L -葡萄糖中培养1天、1周或4周。培养期结束时，在短期孵育中测量基础和刺激状态下的（前）胰岛素及总蛋白合成速率，并测定胰岛胰岛素含量。长时间培养（1 - 4周）后，维持在5.5 mM D -葡萄糖（含或不含L -葡萄糖）的胰岛胰岛素含量增加2.5 - 4倍，而暴露于高葡萄糖的胰岛胰岛素含量基本保持不变。培养1天和1周后，55 mM葡萄糖培养的胰岛对急性葡萄糖刺激的（前）胰岛素生物合成反应不如5.5 mM葡萄糖培养的胰岛明显。这可能主要归因于在高葡萄糖培养的胰岛中观察到的较高基础（前）胰岛素生物合成速率。在培养的第四周结束时，所有三个实验组的（前）胰岛素生物合成速率相似。结果表明，胰岛在体外长时间暴露于异常高的葡萄糖浓度不会对β细胞产生任何特定的有害影响。
