Skavronskaia A G, Gaĭlonskaia I N, Vertiev Iu V, Aleshkin G I, Tiganova I T
Zh Mikrobiol Epidemiol Immunobiol. 1981 Jul(7):22-7.
The possibility of the mobilization of V. cholerae genes responsible for toxin formation (tox gene) and serological specificity (oag-I gene, serotype Inaba) by the RP4::Mu cts62 plasmid was established. The cotransfer of tox and oag-I genes with his and ilv genes respectively by means of the RP4::Mu cts62 plasmid was revealed. The frequency of the cotransfer of tox his and oag-I ilv observed in our experiments corresponded to the known frequency of the cotransfer of these genes observed in crosses with V. cholerae P+-strains. In recombination clones selected according to chromosomal amino acid markers (His+, Ilv+), both carrying and not carrying tox and oag-I genes, no markers of the RP4 plasmid and phage Mu cts62 were detected. The only exception was one His+-recombinant clone. It possessed thermosensitivity (the marker of phage Mu cts62), not linked, however, with sensitivity to phage thermoinduction. Similar clones were obtained when the plasmid was eliminated from V. cholerae strains containing the RP4::Mu cts62 plasmid and having the phenotype corresponding to this plasmid.
已证实RP4::Mu cts62质粒可调动霍乱弧菌中负责毒素形成的基因（tox基因）和血清学特异性的基因（oag-I基因，稻叶型血清型）。通过RP4::Mu cts62质粒分别揭示了tox和oag-I基因与his和ilv基因的共转移情况。在我们的实验中观察到的tox his和oag-I ilv共转移频率与在霍乱弧菌P+菌株杂交中观察到的这些基因的已知共转移频率相对应。在根据染色体氨基酸标记（His+、Ilv+）选择的重组克隆中，无论是否携带tox和oag-I基因，均未检测到RP4质粒和噬菌体Mu cts62的标记。唯一的例外是一个His+重组克隆。它具有热敏感性（噬菌体Mu cts62的标记），但与对噬菌体热诱导的敏感性无关。当从含有RP4::Mu cts62质粒且具有与该质粒相对应表型的霍乱弧菌菌株中消除该质粒时，获得了类似的克隆。
