大肠杆菌中各种蛋白酶的亚细胞分布
Subcellular distribution of various proteases in Escherichia coli.
作者信息
Swamy K H, Goldberg A L
出版信息
J Bacteriol. 1982 Mar;149(3):1027-33. doi: 10.1128/jb.149.3.1027-1033.1982.
Abstract
It has been reported recently that Escherichia coli cells contain eight distinct soluble enzymes capable of degrading proteins to acid-soluble material. Two are metalloproteases that degrade [125I]insulin but not larger proteins: protease Pi, which is identical to protease III, is restricted to the periplasm, and protease Ci is restriction to the cytoplasm. The six others (named Do, Re, Mi, Fa, So, and La, which is the ATP-dependent protease) are serine proteases that degrade [14C]globin and [3H]casein, but not insulin. One of these (Mi) is localized to the periplasm, and one (Re) is distributed equally between the two cellular fractions. The others are present only in the cytoplasm.
摘要
最近有报道称，大肠杆菌细胞含有八种不同的可溶性酶，能够将蛋白质降解为酸溶性物质。其中两种是金属蛋白酶，可降解[125I]胰岛素，但不能降解更大的蛋白质：蛋白酶Pi与蛋白酶III相同，局限于周质，而蛋白酶Ci局限于细胞质。另外六种（分别命名为Do、Re、Mi、Fa、So和La，其中La是ATP依赖性蛋白酶）是丝氨酸蛋白酶，可降解[14C]珠蛋白和[3H]酪蛋白，但不能降解胰岛素。其中一种（Mi）定位于周质，一种（Re）在两个细胞组分中分布均匀。其他几种仅存在于细胞质中。
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