编码蛋白酶III的大肠杆菌ptr基因的完整核苷酸序列。
Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.
作者信息
Finch P W, Wilson R E, Brown K, Hickson I D, Emmerson P T
出版信息
Nucleic Acids Res. 1986 Oct 10;14(19):7695-703. doi: 10.1093/nar/14.19.7695.
Abstract
The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long, which would encode a protein consisting of 962 amino acids with a calculated molecular mass of 107,719 daltons. The predicted primary structure of the protein includes a 23-residue signal sequence, cleavage of which would give rise to a mature protein of molecular mass 105,124 daltons. At its 3' end, the ptr gene overlaps the start of the recB coding sequence by 8 bases, suggesting that these genes may form part of an operon.
摘要
已确定大肠杆菌染色体上一个包含完整ptr基因的3120 bp区域的核苷酸序列。蛋白酶III的推测编码区域长2889个核苷酸，它将编码一种由962个氨基酸组成的蛋白质，计算分子量为107,719道尔顿。该蛋白质的预测一级结构包括一个23个残基的信号序列，信号序列的切割将产生一个分子量为105,124道尔顿的成熟蛋白质。在其3'端，ptr基因与recB编码序列的起始部分重叠8个碱基，这表明这些基因可能构成一个操纵子的一部分。
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