来自人类疟原虫恶性疟原虫的核糖体RNA的分离与鉴定。

Isolation and characterisation of ribosomal RNA from the human malaria parasite Plasmodium falciparum.

作者信息

Hyde J E, Zolg J W, Scaife J G

出版信息

Mol Biochem Parasitol. 1981 Dec 31;4(5-6):283-90. doi: 10.1016/0166-6851(81)90061-x.

Abstract

Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. The overall base composition of the rRNA (40% G+C) is close to that of the rodent parasite Plasmodium berghei, unlike the latter, however, P. falciparum has no nick in the RNA from its large ribosomal subunit.

摘要

从疟原虫恶性疟原虫分离出的核糖体RNA由两种分子量分别为1.49±0.09×10⁶和0.78±0.02×10⁶的物种组成，它们以等摩尔量存在。它们的分子量与其他原生动物的分子量相当，但与人类宿主的分子量截然不同。rRNA的总体碱基组成（40% G+C）与啮齿动物寄生虫伯氏疟原虫的相近，然而，与后者不同的是，恶性疟原虫的大核糖体亚基RNA没有切口。