血清中庆大霉素和妥布霉素三种检测方法的比较评估
Comparative evaluation of three methods for measuring gentamicin and tobramycin in serum.
作者信息
Ngui-Yen J H, Hofmann T, Wigmore M, Smith J A
出版信息
Antimicrob Agents Chemother. 1981 Dec;20(6):821-5. doi: 10.1128/AAC.20.6.821.
Three procedures (radioimmunoassay, fluorescence immunoassay, and enzyme immunoassay) for the determination of gentamicin and tobramycin levels were compared. These systems were evaluated on the basis of accuracy, reproducibility, specificity, and cost. All three systems showed a high degree of accuracy and precision. The fluorescence immunoassay gave significantly lower values for gentamicin and tobramycin at levels below 5 microgram/ml. Of the three systems, the radioimmunoassay was the least expensive, provided that a minimum of 10 analyses were performed daily; single tests required more technical time and were more costly. Costs of single tests by fluorescence immunoassay and enzyme immunoassay systems were similar. The enzyme immunoassay system performed equally well for both gentamicin and tobramycin in giving rapid and accurate results.
对三种测定庆大霉素和妥布霉素水平的方法（放射免疫测定法、荧光免疫测定法和酶免疫测定法）进行了比较。根据准确性、可重复性、特异性和成本对这些系统进行了评估。所有三种系统都显示出高度的准确性和精密度。荧光免疫测定法在庆大霉素和妥布霉素水平低于5微克/毫升时给出的值明显较低。在这三种系统中，放射免疫测定法成本最低，前提是每天至少进行10次分析；单次检测需要更多的技术时间且成本更高。荧光免疫测定法和酶免疫测定法系统的单次检测成本相似。酶免疫测定法系统在快速准确地给出结果方面，对庆大霉素和妥布霉素的检测效果同样良好。
Zotero 插件