从猪身上分离出的大肠杆菌菌株中的甘露糖抗性血凝和定植因子

Mannose-resistant haemagglutination and colonisation factors among Escherichia coli strains isolated from pigs.

作者信息

Oliveira M S, Pestana de Castro A F, Serafim M B, Giorgi W

出版信息

Vet Rec. 1981 Sep 26;109(13):275-8. doi: 10.1136/vr.109.13.275.

DOI:10.1136/vr.109.13.275

Abstract

Ten heat-labile (LT) and 23 heat-stable (ST) enterotoxin-producing strains of enterotoxigenic Escherichia coli (ETEC) and 100 non-enterotoxigenic E coli (non-ETEC) strains isolated from pigs with diarrhoea were examined for the presence of colonisation factors by means of mannose-resistant haemagglutination and serological tests. Seven of 10 LT strains, one of 23 ST and 18 of 100 non-ETEC carried K88 antigen; and four ST and four non-ETEC strains possessed CFA/I antigen. All strains carrying K88, K99 and CFA/I antigens were positive in the mannose-resistant microhaemagglutination (MRMH) test but four ST and 14 non-ETEC isolates positive in the MRMH test did not possess K88, K99 or CFA/I antigens. A considerable number of non-ETEC strains were positive in the MRMH test but lacked K88 and K99 antigens, suggesting that other unknown haemagglutinating colonisation factors may exist in porcine E coli strains.

摘要

采用耐甘露糖血凝试验和血清学试验，对从腹泻猪中分离出的10株产不耐热肠毒素（LT）和23株产耐热肠毒素（ST）的产肠毒素大肠杆菌（ETEC）以及100株非产肠毒素大肠杆菌（non - ETEC）菌株进行了定居因子检测。10株LT菌株中的7株、23株ST菌株中的1株以及100株non - ETEC菌株中的18株携带K88抗原；4株ST菌株和4株non - ETEC菌株拥有CFA/I抗原。所有携带K88、K99和CFA/I抗原的菌株在耐甘露糖微量血凝试验（MRMH）中呈阳性，但4株ST菌株和14株在MRMH试验中呈阳性的non - ETEC分离株不具备K88、K99或CFA/I抗原。相当数量的non - ETEC菌株在MRMH试验中呈阳性，但缺乏K88和K99抗原，这表明猪大肠杆菌菌株中可能存在其他未知的血凝定居因子。